Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Zhai et al. 2025 Raw data
Data includes immunohistochemistry images, two-photon images of patched neurons, whole-cell recordings for somatic excitability and Sr2+-oEPSC, spine density imaging, ACh sensor imaging, and dendritic excitability linescan data.
Autophagy detection assay
Autophagosome detection using Dojindo DAPRed dye and Lysotracker Green in neurons
Ubiquitin enrichment assay
Ubiquitin enrichment assay from neuronal lysates
Immunoprecipitation from primary neurons
Protocol for immunoprecipitating proteins from neuronal lysates
Assay for lysosomal activity using Magic Red
Assay for lysosomal Cathepsin B activity in neurons using Magic Red
Mitophagy detection assay
Detection of mitophagy flux in neurons using Dojindo Mtphagy dye and Lysotracker Green
Lysosomal pH detection assay
Detection of lysosomal pH in live cortical neurons.
Lentiviral transduction
Lentiviral transduction of primary neurons
Data related to “Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy”
Data related to "Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy"
A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation
Phagophore formation requires ATG9, ATG2 and PI3KC3-C1. Authors show that ATG2 is crucial for PI3P appearance on ATG9A compartments with little PI, suggesting a lipid transfer-driven feedback loop supporting lipid transfer and ATG8 lipidation.
Simulated mouse primary motor cortex activity during control and decreased pyramidal tract neuron excitability in the parkinsonian condition
Sixteen files were collected from 4 sets of simulations, each with control and Parkinsonian conditions in rest and activated states. Parkinsonian M1 simulations showed reduced PT5B neuron excitability. Simulations lasted 4.3 seconds.
suny-downstate-medical-center/pd_m1_6ohda: M1_Control_6OHDA
Liqiang Chu et al found decreased excitability in these neurons in a Parkinson's mouse model. A simulation using NetPyNE was created to study the impact of the changed PT5B neuron on motor cortex spiking dynamics.
Derailed degradation: LRRK2-dependent exocytosis in Parkinson’s disease
Ferguson discusses new data on LRRK2 mutations increasing neuronal exocytosis of vesicles enriched in endosomal components. LRRK2 may promote lysosome exocytosis through interactions with GABARAP and Rab GTPases.
Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons
Protocol for depleting or tagging endogenous α-syn using virally-delivered CRISPR-Cas9 in hippocampal neurons
EMPIAR-12885 Datasets associated with AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact
Collection of datasets used in "AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact"
